il 6  (Miltenyi Biotec)

Journal: Annals of Hematology

Article Title: LncRNA PVT1 targets miR-143-3p to modulate endothelial cell function and thereby participate in deep vein thrombosis (DVT) of the lower limbs

doi: 10.1007/s00277-026-06833-4

Figure Lengend Snippet: Effects of si-PVT1 transfection on CoCl 2 –induced angiogenesis and inflammatory levels in HUVECs. A. Changes in vascular endothelial growth factor (VEGF) expression in HUVECs following CoCl 2 induction and si-PVT1 transfection. B. Effects of CoCl 2 induction and LncRNA PVT1 inhibition on fibroblast growth factor-2 (FGF-2) expression in HUVECs. Effects of different transfection treatments on inflammatory cytokine expression: TNF-α (C) and IL-6 (D) . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: We used the following kits to assess the effects of different transfection treatments on endothelial cell angiogenesis and inflammatory levels: the Human VEGF ELISA Kit (97053ES, YENSEN, Shanghai), Human Fibroblast Growth Factor 2 (FGF2) ELISA Detection Kit (JL14546, JONLNBIO, Shanghai), Human TNF-α ELISA Kit (EK182, MULTI SCIENCES, Shanghai), and Human IL-6 ELISA Kit (EK106, MULTI SCIENCES, Shanghai).

Techniques: Transfection, Expressing, Inhibition

Journal: Annals of Hematology

Article Title: LncRNA PVT1 targets miR-143-3p to modulate endothelial cell function and thereby participate in deep vein thrombosis (DVT) of the lower limbs

doi: 10.1007/s00277-026-06833-4

Figure Lengend Snippet: Effects of miR-143-3p inhibitor on HUVEC function and inflammatory levels. Expression of LncRNA PVT1 (A) and miR-143-3p (B) in HUVECs following miR inhibitor transfection. C. Effect of miR inhibitor on viability of damaged HUVECs. D. Apoptotic changes in damaged HUVECs after miR inhibitor transfection. E. Effect of miR inhibitor on apoptosis-related gene expression in damaged HUVECs. F. Changes in VEGF and FG-2 expression in damaged HUVECs following miR inhibitor transfection. G. Effect of miR inhibitor on expression of inflammatory cytokines TNF-α and IL-6 in damaged HUVECs. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: We used the following kits to assess the effects of different transfection treatments on endothelial cell angiogenesis and inflammatory levels: the Human VEGF ELISA Kit (97053ES, YENSEN, Shanghai), Human Fibroblast Growth Factor 2 (FGF2) ELISA Detection Kit (JL14546, JONLNBIO, Shanghai), Human TNF-α ELISA Kit (EK182, MULTI SCIENCES, Shanghai), and Human IL-6 ELISA Kit (EK106, MULTI SCIENCES, Shanghai).

Techniques: Expressing, Transfection, Gene Expression

Journal: bioRxiv

Article Title: Interleukin-6 Regulates the Neutrophil Response to Diverse Bacteria

doi: 10.64898/2026.01.08.698217

Figure Lengend Snippet: (A,C,E) Representative images of extravasated neutrophils preincubated with 0, 10, or 100 ng/mL IL-6 at 0 and 8 hours post stimulation with (A) P. aeruginosa , (C) L. monocytogenes , or (E) S. aureus (scale bar = 250 μm). Yellow lines indicate the edge of the lumen. (B, D, F) Normalized number of extravasated neutrophils in microfluidic devices preincubated with 0, 10, or 100 ng/mL IL-6 responding to (B) P. aeruginosa , (D) L. monocytogenes , or (F) S. aureus . Data quantified from 14 devices for 0 ng/mL, 13 devices for 10 ng/mL, and 10 devices for 100 ng/mL across 4 independent experiments and 3 neutrophil donors for P. aeruginosa . Data quantified from 15 devices for 0 ng/mL, 13 devices for 10 ng/mL, and 13 devices for 100 ng/mL across 4 independent experiments and 3 neutrophil donors for S. aureus . Data quantified from 11 devices for 0 ng/mL, 13 devices for 10 ng/mL, and 12 devices for 100 ng/mL across 4 independent experiments and 3 neutrophil donors for L. monocytogenes . All IL-6 conditions were compared to each other at each time point. Conditions at each time point were compared with a one-way ANOVA and then Tukey’s Honestly Significant Difference (HSD) post-hoc test was used to calculate p-values. Error bars indicate the mean ± SEM. * indicates significance between the 0 and 10 ng/mL conditions at the same time point where p<0.05. † indicates significance between the 0 and 100 ng/mL conditions at the same time point where p<0.05. # indicates significance between the 10 and 100 ng/mL conditions at the same time point where p<0.001.

Article Snippet: After 2 days of culture, 0, 10, 100 ng/mL IL-6 (130-093-929, Miltenyi Biotec) is added to the lumens and incubated at 37 °C and 5.0% CO 2 for approximately 45 minutes.

Techniques:

Journal: bioRxiv

Article Title: Interleukin-6 Regulates the Neutrophil Response to Diverse Bacteria

doi: 10.64898/2026.01.08.698217

Figure Lengend Snippet: (A) Representative images and tracks of migrating neutrophils with 0, 10 or 100 ng/mL IL-6 at the 4-hour timepoint after stimulation with P. aeruginosa or L. monocytogenes (scale bar = 250 μm). Each individual track is shown using different colors. Yellow lines indicate the lumen edge. (B) Neutrophil migration speed and (C) displacement of neutrophils quantified over 10-minute intervals every hour for 8 hours after stimulation with P. aeruginosa . (D) Neutrophil migration speed and (E) displacement of neutrophils quantified over 10-minute intervals every hour for 8 hours after stimulation with L. monocytogenes . Data quantified from 11 devices for 0 ng/mL, 10 devices for 10 ng/mL, and 11 devices for 100 ng/mL across 3 independent experiments and 3 neutrophil donors for P. aeruginosa . Data quantified from 10 devices for 0 ng/mL, 10 devices for 10 ng/mL, and 11 devices for 100 ng/mL across 3 independent experiments and 3 neutrophil donors for L. monocytogenes . Cells were tracked using Cell Motility function in Nikon Elements. All IL-6 conditions were compared to each other at each time point. Conditions at each time point were compared using a one-way ANOVA and then p-values were determined using Tukey’s HSD post-hoc test. Error bars indicate the mean ± SEM. Asterisks indicate significance between conditions at a given timepoint (*p<0.05).

Article Snippet: After 2 days of culture, 0, 10, 100 ng/mL IL-6 (130-093-929, Miltenyi Biotec) is added to the lumens and incubated at 37 °C and 5.0% CO 2 for approximately 45 minutes.

Techniques: Migration

Journal: bioRxiv

Article Title: Interleukin-6 Regulates the Neutrophil Response to Diverse Bacteria

doi: 10.64898/2026.01.08.698217

Figure Lengend Snippet: Representative maximum intensity projections of confocal images of HUVECs seeded in microfluidic devices and incubated with 0, 10, and 100 ng/mL IL-6 for 2 hours (A) with no bacteria and (C) with P. aeruginosa . Cells were fixed and stained with Hoechst (nuclei, blue), phalloidin (actin, red), and anti-VE-cadherin (tight junctions, gray) (scale bar = 250 μm). Images were thresholded in ImageJ to visualize the differences in VE-cadherin. Representative raw images used for protein expression analysis can be found in Figure S2. (B, D) Fold change of VE-cadherin expression was calculated by dividing the total fluorescence intensity of un-thresholded images for each condition by the total fluorescence intensity for the 0 ng/mL IL-6 condition of the corresponding biological replicate. Fold change values for each biological replicate were averaged together and then all IL-6 conditions were compared to each other at each time point. Conditions at each time point were compared using a one-way ANOVA and then p-values were determined using Tukey’s HSD post-hoc test. Error bars indicate the mean ± SEM. (B) Data quantified from 9 devices for 0 ng/mL, 8 devices for 10 ng/mL, and 7 devices for 100 ng/mL across 4 independent experiments for protein expression when not exposed to bacteria. (D) Data quantified from 8 devices for 0 ng/mL, 9 devices for 10 ng/mL, and 8 devices for 100 ng/mL across 3 independent experiments for protein expression when exposed to P. aeruginosa .

Article Snippet: After 2 days of culture, 0, 10, 100 ng/mL IL-6 (130-093-929, Miltenyi Biotec) is added to the lumens and incubated at 37 °C and 5.0% CO 2 for approximately 45 minutes.

Techniques: Incubation, Bacteria, Staining, Expressing, Fluorescence

Journal: bioRxiv

Article Title: Interleukin-6 Regulates the Neutrophil Response to Diverse Bacteria

doi: 10.64898/2026.01.08.698217

Figure Lengend Snippet: Representative maximum intensity projections of confocal images of HUVECs seeded in microfluidic devices and incubated with 0, 10, and 100 ng/mL IL-6 for 2 hours (A) with no bacteria and (C) with P. aeruginosa . Cells were fixed and stained with Hoechst (nuclei, blue), phalloidin (actin, red), and anti-ICAM-1 (neutrophil tight binding to endothelial cells, green) (scale bar = 250 μm). Images were thresholded in ImageJ to visualize the differences in ICAM-1. Representative raw images used for protein expression analysis can be found in Figure S2. (B, D) Fold change of ICAM-1 expression was calculated by dividing the total fluorescence intensity of un-thresholded images for each condition by the total fluorescence intensity for the 0 ng/mL IL-6 condition of the corresponding biological replicate. Fold change values for each biological replicate were averaged together and then all IL-6 conditions were compared to each other at each time point. Conditions at each time point were compared using a one-way ANOVA and then p-values were determined using Tukey’s HSD post-hoc test. Error bars indicate the mean ± SEM. (B) Data quantified from 9 devices for 0 ng/mL, 8 devices for 10 ng/mL, and 7 devices for 100 ng/mL across 4 independent experiments for protein expression when not exposed to bacteria. (D) Data quantified from 8 devices for 0 ng/mL, 9 devices for 10 ng/mL, and 8 devices for 100 ng/mL across 3 independent experiments for protein expression when exposed to P. aeruginosa .

Article Snippet: After 2 days of culture, 0, 10, 100 ng/mL IL-6 (130-093-929, Miltenyi Biotec) is added to the lumens and incubated at 37 °C and 5.0% CO 2 for approximately 45 minutes.

Techniques: Incubation, Bacteria, Staining, Binding Assay, Expressing, Fluorescence